Gene targeting strategy for B-hIL2RB mice. The exons 2~8 of mouse Cd122 gene that encodes the extracellular domain were replaced by human CD122 exons 2~8 in B-hIL2RB mice.

Strain specific IL2RB expression analysis in heterozygous B-hIL2RB mice by flow cytometry. Splenocytes were collected from WT mice and heterozygous B-hIL2RB mice (H/+) stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-IL2RB antibody. Mouse IL2RB was detectable in WT and heterozygous B-hIL2RB mice. Human IL2RB was exclusively detectable in heterozygous B-hIL2RB mice but not in WT mice.