C57BL/6-Cd3etm2(CD3E)Bcgen Folh1tm1(FOLH1)Bcgen/Bcgen • 112608
Gene targeting strategy for B-hCD3E/hPSMA mice. The exons 2-6 of mouse Cd3e gene that encode the extracellular domain were replaced by human CD3E exons 2-7 in B-hCD3E/hPSMA mice.The extracellular coding sequence of human PSMA gene and mouse 3’ UTR were inserted into mouse Psma exon 2 in B-hPSMA mice, so the mouse Psma gene was disrupted. The intracellular and transmembrane sequence of mouse Psma did not change.
Immunohistochemical (IHC) analysis of PSMA expression in homozygous B-hCD3E/hPSMA mice. Various tissue lysates were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hCD3E/hPSMA mice (H/H; H/H), and then and analyzed by IHC with anti-PSMA antibody (abcam, ab133579). PSMA was detectable in the brain and kidney of wild-type mice and B-hCD3E/hPSMA mice due to the cross-reactivity of antibody.